Figure 1.

Alterations in SJs in Posterior Midguts of Smurf and Non-Smurf Flies

(A–E) SJ protein localization in ECs in Smurf or Non-Smurf midguts in 13-day-old w¹¹¹⁸ female flies. Representative images (A) and SJ/cytoplasm fluorescence ratios (B–E) for Ssk, Dlg, Mesh, and Coracle. SJ protein mislocalization is observed in Smurf ECs, represented by an increase in cytoplasmic localization. n ≥ 14 midguts per condition; n = 10 ECs were observed per midgut; scale bar, 5 μm.

(F–J) SJ protein localization in ECs in Smurf or non-Smurf midguts in 30-day-old w¹¹¹⁸ female flies. Representative images (F) and SJ/cytoplasm fluorescence ratios (G–J) for Ssk, Dlg, Mesh, and Coracle. SJ protein mislocalization is observed in both Smurf and non-Smurf ECs, represented by an increase in cytoplasmic localization. n ≥ 14 midguts per condition; n = 10 ECs were observed per midgut; scale bar, 5 μm.

Samples were dissected and stained in parallel under the same conditions; pictures taken at same laser intensity. Data analyzed utilizing a two-tailed unpaired Student's t test, and the error bars are the SEM range of those averages. *p < 0.05; ***p < 0.001; ****p < 0.0001 represent a statistically significant difference. See also Figure S1.