Table 1.

SAXS data collection and analysis parameters

SAS data collection parameters
Instrument	BioCAT facility at the Advanced Photon Source beamline 18ID with Pilatus3 1M (Dectris) detector
Wavelength (Å)	1.033
Beam size (μm2)	160 (h) × 75 (v)
Camera length (m)	3.5
q measurement range (Å−1)	0.00365–0.383
Absolute scaling method	NAa
Basis for normalization to constant counts	To incident intensity, by ion chamber counter
Method for monitoring radiation damage	Automated frame-by-frame comparison of relevant regions
Exposure time, number of exposures	1-s exposure time with a 2-s total exposure period of entire SEC elution
Sample configuration	SEC-SAXS. Size separation by an ÄKTA Pure with a Superdex 200 Increase 10/300 GL column. SAXS data measured in a 1.5-mm inner diameter quartz capillary.
Sample temperature (°C)	20
Software employed for SAS data reduction, analysis, and interpretation
SAXS data reduction	Radial averaging; frame comparison, averaging, and subtraction done using BioXTAS RAW 1.4.0 (55) and ATSAS (56)
Basic analysis: Guinier, molecular weight, P(r)	Guinier fit and molecular weight using BioXTAS RAW 1.4.0 (55), P(r) function using GNOM (65)

^a Not applicable.