Figure 4.

Seeding properties of fibrils composed of htau40 truncated at both N and C termini. 10% of fibril seeds were mixed with 10 μm Tau monomers and allowed to grow for 20–24 h at 37 °C. The fibrils were sedimented and analyzed by SDS–PAGE and Coomassie staining. A–F, representative gels (left panels) and densitometric quantification depicted as box-and-whisker plots using the Tukey method (right panels). The horizontal line inside the box represents the median. P, pellet; S, supernatant; n, number of biological replicates. Statistical comparisons were performed using paired t tests. **, p ≤ 0.01; ****, p ≤ 0.0001. The data reveal that successive shortening of the C terminus of htau40 enhances the cross-seeding barrier with htau23.