Skip to main content
. 2018 Sep 26;293(45):17418–17429. doi: 10.1074/jbc.RA118.004432

Figure 4.

Figure 4.

Interaction of WT and mutant E163 proteins with the cellular surface. Cells were incubated with 250 nm of the indicated recombinant purified proteins and bound E163 protein was detected with a polyclonal anti-E163 antibody by flow cytometry. A, histograms show representative binding to CHO-K1 cells of the recombinant proteins: E163 (blue), E163-1 to E163-6 (green), negative control without protein (gray), B18 (pink), and M3 (cyan). B, scatter plots show the binding, mean (horizontal lines) and standard deviation (S.D., vertical lines), of the proteins to CHO-K1 cells in each case (n = 3). The asterisk indicates significant differences (p < 0.05) with respect to E163 protein binding. C, histograms show representative binding to CHO-618 cells of the recombinant proteins: E163 (blue), E163-1 to E163-6 (green), negative control without protein (gray), B18 (pink), and M3 (cyan). D, scatter plots show the binding, mean (horizontal lines) and S.D. (vertical lines), of the proteins to CHO-618 cells in each case (n = 3). Data shown are representative of three experiments.