Figure 4.

IL-27 partially affects LPS binding in tolerized THP-1 cells but not in PMA–THP-1 cells, primary monocytes, or primary macrophages. THP-1 cells (A), PMA–THP-1 cells (B), primary monocytes (C), and primary macrophages (D) were stimulated with LPS (10 ng/ml), IL-27 (100 ng/ml), or LPS + IL-27 simultaneously for 24 h with or without BB-94 (25 μm). Cells were subsequently incubated with LPS-Alexa (100 ng/ml) for 1 h at 37 °C. Bound LPS was measured by flow cytometry. The -fold changes were calculated for LPS-, IL-27-, and LPS + IL-27–treated cells relative to medium controls. Graphs present data from at least three independent experiments.