Figure 7.

Overexpression of CD14 in THP-1 cells does not alter IL-27–mediated inhibition of endotoxin tolerance. CD14-THP-1 cells (A) and PMA–CD14-THP-1 cells (B) were stimulated with LPS (10 ng/ml), IL-27 (100 ng/ml), or LPS + IL-27 simultaneously for 24 h (1°). Cells were washed and challenged with LPS (100 ng/ml) for 4 h (2°). TNF-α production was measured in cell-free supernatants by ELISA. The -fold change of TNF-α production was calculated relative to untolerized (1° med/2° LPS) cells. Graphs present mean ± S.E. of at least six independent experiments.