Figure 2.

Enzymatic properties of PpLGDH. A, thermal stability after incubation of the enzyme for 30 min at pH 7.5. B, pH stability after incubation of the enzyme for 30 min at 40 °C. C, effect of temperature on the activity (forward reaction). D, s–v plot of the forward reaction with 2 mm NAD⁺ at pH 8.5. E, s–v plot of the reverse reaction with 0.2 mm NADH at pH 7.0. F, effect of pH on the forward reaction with 20 mm LG and 2 mm NAD⁺. G, effect of pH on of the reverse reaction with 2 mm 3-keto-LG and 0.2 mm NADH. See “Experimental procedures” for detailed conditions.