Figure 9.

Complex formation of SMS1–GCS alters sensitivity to CERT inhibitor HPA-12. HEK293T, GCS KO, and SMS1 KO cells were pretreated with HPA-12 at the indicated concentrations in the growth medium for 1 h and further cultivated with 0.5 μCi of [¹⁴C]stearic acid (SA) for 3 h. Lipids were extracted, saponified, and separated by TLC (A and D). The values of SM (B), Cer (C and F), and GlcCer (E) are expressed as percentages of radioactive SM, Cer, or GlcCer to vehicle-treated control cells and represent the means ± S.D. from three independent experiments. *, p < 0.01; **, p < 0.05.