CARM1 directly interacts with PRMT5.
A, Western blot analysis of PRMT5 binding to purified GST or GST-CARM1 using PRMT5 antibody (top). SDS-PAGE analysis of purified GST or GST-CARM1 from E. coli stained by Coomassie Brilliant Blue (CBB) (bottom). B, Western blot analysis of CARM1 binding to purified GST, GST-PRMT5(1–637), GST-PRMT5(1–354), GST-PRMT5(355–453) or GST-PRMT5(454–637) using CARM1 antibody (top). SDS-PAGE analysis of purified GST, GST-PRMT5(1–637), GST-PRMT5(1–354), GST-PRMT5(355–453), or GST-PRMT5(454–637) from E. coli stained by Coomassie Brilliant Blue (CBB) (bottom). Black asterisks denote the GST fusion proteins in assays. C, coimmunoprecipitation of PRMT5 and CARM1 from FLAG-PRMT5 overexpressing Lys-562 cell lysate. IgG was used as the negative control.