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. 2018 Sep 26;293(45):17454–17463. doi: 10.1074/jbc.RA118.004028

Figure 6.

Figure 6.

CARM1 methylates PRMT5 Arg-505 to repress γ-globin expression. A and C, quantitative real-time PCR analysis of CARM1 (A) and γ-globin (C) mRNA levels normalized to β-actin in Lys-562 cells overexpressing PRMT5-WT, PRMT5-R505A, or PRMT5-R505K with or without CARM1 knockdown. The results are shown as the mean ± S.D. from three independent experiments. Two-tailed Student's t-tests were used to compare means. **, p < 0.01, #, p > 0.05 compared with indicated controls. B, Western blot analysis of indicated protein levels in cell lysates from Lys-562 cells overexpressing PRMT5-WT, PRMT5-R505A, or PRMT5-R505K with or without CARM1 knockdown. GAPDH and histone H3 were used as negative controls. D, ChIP analysis of H4R3me2s and H3R17me2a enrichment at the γ-promoter in Lys-562 cells overexpressing PRMT5-WT, PRMT5-R505A, or PRMT5-R505K with or without CARM1 knockdown. The results are shown as the mean ± S.D. from three independent experiments.