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Arg-505 methylation is essential for PRMT5 oligomerization. A and B, Western blot analysis using PRMT5 antibody to detect PRMT5 oligomerization in Lys-562 cells overexpressing PRMT5-WT, PRMT5-R505A, or PRMT5-R505K treated with PBS control (A) or the cross-linker EDC+NHS (B). The asterisk and the number sign indicate the PRMT5 dimer and PRMT5 tetramer, respectively.
