Fig. 2.

Impact of TERT promoter mutations on telomere-associated parameters in meningiomas. (A) Telomerase activity was determined by the TRAP assay and results were expressed as total product generated units (TPG). (B) TERT mRNA expression levels were analyzed by semiquantitative RT-PCR (relative to glyceraldehyde 3-phosphate dehydrogenase mRNA) and (C) telomere lengths by real-time PCR (relative to the alternative lengthening of telomeres‒positive osteosarcoma cell line SA-OS) in TERTp-wt and TERTp-mut meningioma subgroups. Statistical analyses were performed by Student’s t-test, and significance (P < 0.001) was expressed using 3 asterisks.