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miR-186-5p affected IGF-1 expression in OGD/R-treated SH-SY5Y cell. SH-SY5Y cells in OGD/R group were treated with miR-NC, miR-186-mimics or miR-186 inhibitor. (A) IGF-1 mRNA expression was detected by qRT-PCR. (B) Tthe protein expression of IGF-1 was analyzed by western blots. GAPDH was used as internal control. (C) IFG-1 was quantified by ELISA. *P < 0.05, **P < 0.01, ***P < 0.001 versus control or miR-NC group; results are representative of three separate independent experiments.
