Figure 3.

Knockdown of P16 rescued SF- and SC-EMSC from senescence. (A) P16 mRNA expression in an indicated passage of SF- and SC-EMSC was detected by RT-PCR. GAPDH was used as a loading control. (B) SF- and SC-EMSC were transduced with lentivirus co-expressing GFP and shP16 or shNC, GFP⁺ cells were sorted and tested for P16 expression via RT-PCR. GAPDH was used as a loading control. (C) P16 protein in GFP⁺ SF- and SC-EMSC was detected via western blotting. GAPDH was used as a loading control. (D) The senescence of GFP⁺ SF- and SC-EMSC were detected by senescence-associated beta-galactosidase assays. Total cell number was counted by DAPI staining. (E) Cell proliferation was evaluated GFP⁺ SF- and SC-EMSC using the CCK-8 assay. *P < 0.05, **P < 0.01.