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. 2018 Oct 20;14(13):1883–1891. doi: 10.7150/ijbs.27854

Figure 6.

Figure 6

Bcl-2 and Bax modulate the cell death induced by shikonin. (A) T24 resistant cells were transfected with Bcl-2 siRNA, and subsequently treated with 0.4 μM shikonin (SKN) and 25 µM cisplatin (Cis) alone or their combination (C+S) for 24 h. The expression of indicated proteins was analyzed by western blot. (B and C) The T24 resistant cells were treated as in (A). B, the cell viability was analyzed by MTT assay. C, the apoptosis was analyzed by Hoechst 33258 staining. (D) T24 resistant cells were transfected with Bax plasmid, and subsequently treated with 0.4 μM shikonin and 25 µM cisplatin alone or their combination for 24 h. The expression of indicated proteins was analyzed by western blot. (E and F) The T24 resistant cells were treated as in (D). E, the cell viability was analyzed by MTT assay. F, the apoptosis was analyzed by Hoechst 33258 staining. N, p>0.05; **, p<0.01, ***, p<0.001.