Figure 1.

SIRT3 decreases in mouse OA cartilage and TNF-α-treated rat chondrocytes (A, B) Double meniscus destabilization resulted in articular cartilage necrosis and thining. Scale bar 50 μM. (C) Immunofluorescence staining of SIRT3 in rat knee articular cartilage from sham group and OA group. (D, E) SIRT3 levels of chondrocytes from human cartilage. (F, G) The level of SIRT3 in chondrocytes treated with different concentrations of TNF-α for 24 h. (H, I) The level of SIRT3 in chondrocytes treated with TNF-α (50 ng/ml) for increasing time (0, 6 h, 12 h and 24 h). All experiments were performed as mean ± SD of 5 times in duplicates. *P < 0.05, **P < 0.01.