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. 2018 Oct 20;14(13):1873–1882. doi: 10.7150/ijbs.27746

Figure 4.

Figure 4

DHM promotes the capacity of anti-oxidation via activating SIRT3 in rat chondrocytes. (A, B) Representative micrographs of MitoSOX assay showed the effects of DHM on the ROS level in chondrocytes against TNF-α. Scale bar 100 μM.(C, D) Representative western blots and results of densitometry analysis of acetyl-k68 MnSOD in rat chondrocytes treated as above. (E) DHM promoted the activity of MnSOD via enhancing SIRT3 in chondrocytes. All experiments were performed as mean ± SD of 5 times in duplicates. *P < 0.05, **P < 0.01.