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. Author manuscript; available in PMC: 2018 Nov 12.
Published in final edited form as: J Am Chem Soc. 2018 Sep 11;140(38):12102–12110. doi: 10.1021/jacs.8b06738

Figure 2.

Figure 2.

Inhibition of the NEMO-IKKα/β interaction and NF-κB signaling by peptide 7 and control peptides. (a) Inhibition of the NEMO-IKKβ interaction as monitored by the HTRF assay. (b) Cellular uptake efficiency of FITC-labeled peptides into HeLa cells as determined by flow cytometry. All values are relative to that of CPP1 (100%). (c) Differential effects of peptide 7 on the basal (open bars) and TNFα-induced NF-κB activation (closed bars) in HEK293(Luc) cells. *, p < 0.001 using Student’s t test. (d) Comparison of peptides 1, 7, 20, and 21 for inhibition of TNFα-induced luciferase activity in HEK293(Luc) cells. (e) Western blot showing the effect of peptide 7 on IκBα and IKKβ levels in HT29 colon cancer cells in the absence and presence of TNFα.