Figure 7.
Expression patterns of biocontrol-associated genes after exposure to F. oxysporum VCs. Transcripts from 11 genes in (A) T. virens strain G-41 and (B) T. harzianum strain T-22 were quantified after co-cultivation with four F. oxysporum strains (NRRL26379, NRRL38499, NRRL38487, and NRRL37616) for 40 h and 33 h, respectively. Transcripts from the elongation factor 1-α and β-actin genes were used as internal references to normalize the level of transcripts for individual genes. Control corresponded to Trichoderma co-cultivated with un-inoculated PDA. Values shown correspond to the relative expression ± SD of data from three replicates. Gene identity based on the abbreviation scheme shown in Table 2 is indicated. * indicates up-regulation (relative expression level >2) or down-regulation (relative expression level < 0.5).