Table 2.
Trichoderma genes chosen for expression analysis.
| Gene (abbreviation)a | Function | Mode of inductionb | Homologous gene in ref. genomec | |
|---|---|---|---|---|
| T. virens | T. harzianum | |||
| chitinase 33 (chit) | Cell wall degradation | AC | 189743 | 529621 |
| endochitinase 42 (endo) | Cell wall degradation | BC | 218832 | 101028 |
| β-1,3-endoglucanase (bgn) | Cell wall degradation | AC | 231316 | 485240 |
| trypsin-like protease pra1 (pra) | Protein degradation | AC | 181449 | 526221 |
| subtilisin-like protease prb1 (prb) | Protein degradation | AC | 186844 | 110777 |
| QID74 protein (qid) | Cell wall integrity | BC | 230780 | 509414 |
| small heat-shock protein Hsp26 (hsp) | Posttranslational processing | AC | 195080 | 8037 |
| acyl-CoA synthetase (acs) | Lipid metabolism | BC | 203203 | 479794 |
| anthranilate synthase component II (ans) | Amino acid metabolism | BC | 193889 | 534341 |
| polyketide synthase (pks) | Secondary metabolism | AC | 204589 | 521432 |
| terpene synthase (tps) | Terpene synthesis | unknown | 141474 | 523651 |
| translation elongation factor 1-α (tef) | Housekeeping gene | – | 216092 | 12328 |
| β-actin (act) | Housekeeping gene | – | 229062 | 510028 |
a
With the exception of tps, tef, and act, they were chosen because their expression was reported to be induced during mycoparasitism.
b
AC, gene expression induced after physical contact. BC, gene expression induced both before and after physical contact. –, no induction.
c
Transcript IDs of the genes in T. virens Gv29-8 v2.0 and T. harzianum CBS 226.95 v1.0 are shown.