Figure 7.

Cachd1 Competes with α₂δ-1 for Effects on Ca_V2.2 Currents

(A) Mean (± SEM) current-voltage relationships for WT Ca_V2.2-HA co-expressed with β1b and either α₂δ-1 (red solid circles, n = 25), Cachd1 (green solid circles, n = 18), or α₂δ-1 and Cachd1 (purple solid circles, n = 18). The individual and mean data were fit with a modified Boltzmann equation (STAR Methods).

(B) G_max (nS/pF) from the current-voltage relationships shown in (A). Individual data (same symbols as in A) and mean ± SEM are plotted. ^∗p = 0.0483, ^∗∗p = 0.0086 (1-way ANOVA with Holm-Sidak’s post hoc test correcting for multiple comparisons).

(C) Mean (± SEM) current-voltage relationships for D122A Ca_V2.2-HA co-expressed with β1b and either α₂δ-1 (red open circles, n = 10), Cachd1 (green open circles, n = 8), or α₂δ-1 and Cachd1 (purple open circles, n = 12).

(D) G_max (nS/pF) from the current-voltage relationships shown in (C). Individual data (same symbols as in C) and mean ± SEM are plotted. ^∗p = 0.0342, †p = 0.0265 (1-way ANOVA with Holm-Sidak’s post hoc test correcting for multiple comparisons).

(E and F) Bar charts of mean (± SEM) V_rev (millivolt) for the conditions shown in (A) and (C), respectively. ^∗p = 0.0396, ^∗∗p = 0.0088, ^∗∗∗p = 0.0002, ^∗∗∗∗p < 0.0001 (1-way ANOVA with Holm-Sidak’s post hoc test correcting for multiple comparisons).