REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
α2δ-1 Ab	Sigma-Aldrich	Cat # C5105; RRID:AB_258885
Anti-CaV2.2 II-III loop Ab (rabbit polyclonal)	(Raghib et al., 2001)	n/a
Anti-HA Ab rat monoclonal	Sigma-Aldrich	Cat# 11815016001; RRID:AB_390914
Anti-HA Ab rabbit	Sigma-Aldrich	Cat # H6908; RRID:AB_260070
Anti-GAPDH Ab	Ambion	Cat # AM4300; RRID:AB_2536381
Anti-GFP Ab (Living Colors, rabbit polyclonal)	Takara Bio Clontech	Cat # 632375
Anti-rabbit Alexa fluor 594	Thermo Fisher	Cat # R37117; RRID:AB_2556545
Anti-rat Alexa fluor 488	Thermo Fisher	Cat # A-11006; RRID:AB_2534074
Anti-mouse Alexa fluor 647	Thermo Fisher	Cat # A32728; RRID:AB_2633277
Anti-rat fluorescein isothiocyanate	Sigma-Aldrich	Cat # F1763; RRID:AB_259443
Goat anti-rabbit HRP	Biorad	Cat # 1706515; RRID:AB_11125142
Goat anti-rat HRP	Biorad	Cat # 5204-2504; RRID:AB_619913
Goat anti-mouse HRP	Biorad,	Cat # 1721011; RRID:AB_11125936
Affinity-purified Cachd1 rabbit polyclonal Ab	G. T. Powell and S.W Wilson, UCL.	n/a
Chemicals, Peptides, and Recombinant Proteins
ω-conotoxin GVIA	Alomone	Cat # C-300
Penicillin-Streptomycin (10,000 U/mL)	Invitrogen	Cat # 15140-122
Poly-L-lysine	Sigma-Aldrich	Cat # P.6282
Dulbecco’s modified Eagle’s medium	Thermo Fisher	Cat #4 1965-039
GlutaMAX	Invitrogen	Cat # 35050-038
Fugene	Promega	Cat # E2311
Polyjet	Tebu-bio Ltd	Cat # 189-SL100688-1
Opti-MEM	Thermo Fisher	Cat # 41965-039
Neurobasal Medium	Invitrogen	Cat # 10888-022
B27	Thermo Fisher	Cat # 17504044
HEPES	Sigma-Aldrich	Cat # H3375
Horse serum	Invitrogen	Cat # 26050-088
Lipofectamine 2000	Invitrogen	Cat # L3000-008
Premium Grade EZ-link Sulfo-NHS-LC-Biotin	Thermo Fisher	Cat # 21335
Glycine	Sigma-Aldrich	Cat # G8898
SDS	VWR	Cat # 444062F
Protease Inhibitors	Roche	Cat # 11697498001
DTT	Melford	Cat # MB1015
SDS-polyacrylamide gel electrophoresis	Invitrogen	Cat # EA0375BOX
polyvinylidene fluoride (PVDF) membrane	Biorad	Cat # 1620177
streptavidin-agarose beads	Thermo Fisher	Cat # 20347
Igepal	Sigma-Aldrich	Cat # I3021
PNGase-F	Roche Applied Science	Cat # 11365177001
Digitonin	Millipore	Cat # 300410
A/G PLUS Agarose slurry Santa Cruz	Santa Cruz	Cat # Sc-2003
Paraformaldehyde	Sigma-Aldrich	Cat # P6148
Goat serum	Invitrogen	Cat # 6210-072
Triton X-100	Thermo Fisher	Cat # 28314
4’,6-diamidine-2′-phenylindole dihydrochloride (DAPI)	Molecular probes	Cat # nl5995050
VectaShield	Vector Laboratories	Cat # H1000
papain	Sigma-Aldrich	Cat # P4762
L-cysteine	Sigma-Aldrich	Cat # c7755
bovine serum albumin	First Link UK ltd	Cat # 41-00-410
DNase	Sigma-Aldrich	Cat # D5025
Hank’s basal salt solution	Thermo Fisher	Cat # 14175-053
α-bungarotoxin Alexa Fluor® 488 conjugate (BTX488)	Thermo Fisher	Cat # B13422
fetal bovine serum	Invitrogen	Cat # 10270
Critical Commercial Assays
Bradford Assay	Biorad	Cat # 500-0006
ECL 2	Thermo Fisher	Cat # 32132
Experimental Models: Cell Lines
tsA-201 cells	ECACC	Cat # 96121229
Neuro2A cells	ATCC	CCL-131
Experimental Models: Organisms/Strains
Rat Sprague Dawley male	UCL bred in house	n/a
Oligonucleotides
Primer for introducing the D122A mutation. reverse 5′-GACATAGGCGTCTTGGCCCCGTCAG-3′	this paper	n/a
Primer for introducing the D122A mutation forward 5′-CTGACGGGGCCAAGACGCCTATGTC-3′	this paper	n/a
Recombinant DNA
Rabbit CaV2.2 HA	(Cassidy et al., 2014)	n/a
Rat β1b (X61394)	(Pragnell et al., 1991)	n/a
Rat α2δ-1 (M86621)	(Kim et al., 1993)	n/a
HA tagged α2δ-1	(Kadurin et al., 2012)	n/a
Rat CaV2.1	(Brodbeck et al., 2002)	n/a
Human TASK3 (KCNK9) (NM_001282534)	obtained from Prof. A Mathie	n/a
Zebrafish zCachd1	G. T. Powell and S.W Wilson, UCL.	n/a
Rat rCachd1	OriGene	Cat # RN217577
GFP_CaV2.2-HA	(Macabuag and Dolphin, 2015)	n/a
CaV2.2-BBS	(Cassidy et al., 2014)	n/a
CaV2.2-HA D122A	This paper	n/a
GFP_CaV2.2-HA D122A	This paper	n/a
rCachd1_GFP	This paper	n/a
zCachd1_GFP	G. T. Powell and S.W Wilson, UCL.	n/a
Mouse α2δ-3 (AJ010949)	(Klugbauer et al., 1999)	n/a
mcherry (AY678264)	(Shaner et al., 2004)	n/a
CD8	(Shy et al., 2014)	n/a
Software and Algorithms
ImageJ	National Institutes of Health https://imagej.nih.gov/ij/	RRID:SCR_003070
GraphPad Prism 5 or 7	https://www.graphpad.com	n/a
Origin-Pro 2015	Microcal Origin, Northampton, MA	n/a
pCLAMP 9	Molecular Devices	n/a