Figure 4.

The YAP Interactome Is Altered in Cav1KO MEFs

(A and B) Mass spectrometry analysis of YWHA proteins (A) and nuclear pore components and nucelocytosolic transporters (B) that co-immunoprecipitate with YAP in WT and Cav1KO MEFs treated with or without 1 μM CytD for 24 hr. The heatmap represents the relative number of counts per protein and condition. Negative controls (first and third column) were performed in parallel by omitting the primary anti-YAP antibody. n = 5.

(C) siRNA library screening scheme for identifying YAP activity regulators.

(D) Plot of mean Z scores of the YAP nuclear-to-cytosolic ratio for each individual siRNA in WT and Cav1KO MEFs.

(E) Mean Z score of the YAP nuclear-to-cytosolic ratio in WT and Cav1KO cells after siRNA transfection for those genes whose Z scores are above 2.5 or below −2.5. n = 3.

(F) qRT-PCR of Ctgf and Ankrd1 in cells transfected with control or YWHAH siRNAs: WT and Cav1KO MEFs, WT cells treated for 24 hr with 1 μM CytD, and WT cells grown on soft substrate. n = 5. Data are presented as means ± SEM. ^∗p < 0.05 and ^∗∗p < 0.01.

(G) Scheme of CAV1-YAP regulation.

See also Figures S4 and S5, Table S2, and Data S1.