REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-YAP (63.7)	Santa Cruz Biotechnology	Cat# sc-101199, RRID: AB_1131430
Mouse monoclonal anti-TEF-1 (H-4)	Santa Cruz Biotechnology	Cat# sc-376113, RRID: AB_10988229
Rabbit monoclonal anti-CAV1 (D46G3) XP	Cell Signaling Tecnology	Cat# 3267S, RRID: AB_2275453
Rabbit monoclonal anti-14-3-3 η (D23B7)	Cell Signaling Tecnology	Cat# 5521S, RRID: AB_10829034
Monoclonal ANTI-FLAG® M2 antibody	Sigma-Aldrich	Cat# F3165, RRID: AB_259529
Mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase antibody	Millipore	Cat# MAB374, RRID: AB_2107445
Rabbit polyclonal anti-Phospho-YAP (Ser127) Antibody	Cell Signaling Technology	Cat# 4911S, RRID: AB_2218913
Rabbit polyclonal anti-Anti-LATS1 Antibody	Cell Signaling Technology	Cat# 9153S, RRID: AB_2296754
Rabbit polyclonal anti-Anti-LATS1 Antibody	Cell Signaling Technology	Cat#13646
Rabbit polyclonal anti-Anti-H3 Histone Antibody	Abcam	Cat# ab1791, RRID: AB_302613
Rabbit polyclonal anti-RHO GDIalpha (A-20) antibody	Santa Cruz Biotechnology	Cat# sc-360, RRID: AB_2227516
Rabbit monoclonal anti-YAP (D8H1X) XP	Cell Signaling Technology	Cat# 14074, RRID: AB_2650491
Rabbit Anti-Human Actin, Smooth Muscle Epitope Specific antibody	Thermo Fisher Scientific	Cat# RB-9010-P0, RRID: AB_149755
Alexa Fluor 488 Goat Anti Mouse IgG (H+L)	Molecular Probes	Cat# A-11029, RRID: AB_138404
Alexa Fluor 546 Goat Anti Mouse IgM (m chain)	Molecular Probes	Cat# A-21045, RRID: AB_2535714
Alexa Fluor 546 Goat Anti Rabbit IgG (H+L)	Molecular Probes	Cat# A-11035, RRID: AB_143051
Alexa Fluor 488 Goat Anti Rabbit IgG (H+L)	Molecular Probes	Cat# A-11034, RRID: AB_2576217
Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP	Thermo Fisher Scientific	Cat# 31430, RRID: AB_228307
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP	Thermo Fisher Scientific	Cat# 31460, RRID: AB_228341
Bacterial and Virus Strains
N/A	N/A	N/A
Biological Samples
N/A	N/A	N/A
Chemicals, Peptides, and Recombinant Proteins
ROCK inhibitor Y27632	Sigma-Aldrich	Y0503; CAS Number 129830-38-2
CytochalasinD	Sigma-Aldrich	C8273; CAS Number: 22144-77-0
Jasplakinolide	Santa Cruz Biotechnology	sc-202191; CAS 102396-24-7
Caerulein	Sigma-Aldrich	C9026; CAS Number: 17650-98-5
Critical Commercial Assays
Lipofectamine RNAiMAX Transfection Reagent	Invitrogene	Cat#10601435
Alexa Fluor 647 Phalloidin	Invitrogene	Cat# A22287, RRID:AB_2620155
Dual-Luciferase Reporter Assay System	Promega	Cat#E1910
RNAeasy micro kit	QIAGEN	Cat#74004
Omniscript RT kit	QIAGEN	Cat# 205111
Random primers	Promega	Cat#C1181
SYBR green	Applied Biosystems	Cat#4309155
G-Actin/F-actin In Vivo Assay Biochem Kit	Cytoskeleton	Cat#BK037
Deposited Data
RNaseq data	This paper	GEO: GSE120514
Mass spectrometry data	Table S2	N/A
Experimental Models: Cell Lines
WT and Cav1KO Mouse embryonic fibroblasts (MEF)	Razani et al., 2001	N/A
WT and Cav1KO Neonatal Hepatocytes (mouse)	Mayoral et al., 2010	N/A
MDA-MB-231 HTB-26 breast adenocarcinoma (human)	ATCC	Cat# HTB-26, RRID: CVCL_0062
HeLa CCL-2	ATCC	Cat# CCL-2, RRID: CVCL_0030
HeLa Cav1–GFP	Lukas Pelkmans laboratory	N/A
Primary pancreatic cancer associated fibroblasts (PanCAF)	Manuel Hidalgo Laboratory	N/A
Experimental Models: Organisms/Strains
Mouse: Cav1KO C57BL/6	Drab et al., 2001	N/A
Oligonucleotides
siRNA targeting sequence: Cav1 #1: GAGCUUCCUGAUUGAGAUU (sense sequence)	This paper	N/A
ON-Target Plus Smart-pool siRNAs; see Table S3	Dharmacon	N/A
Primers for qRT-PCR	Table S4	N/A
Recombinant DNA
p2xFlag CMV2-YAP2	Oka et al., 2008	Addgene #19045
pCMV-flag YAP2 5SA	Zhao et al., 2007	Addgene #27371
8xGTIIC-luciferase	Dupont et al., 2011	Addgene #34615
pLVX-CMV-CherryFP-P2A-MetLuc	This paper	N/A
pLVX_shRNA2	Clontech	Cat#632179
CMVCherryFPP2A	Viral vectors Unit (CNIC)	N/A
pMetLuc reporter	Viral vectors Unit (CNIC)	N/A
pEGFP–mDia1(DeltaN3)	Ishizaki et al., 2001	N/A
pEGFP-RHO(V14)	del Pozo et al., 1999	N/A
Software and Algorithms
qBase plus	Biogazelle	N/A
MATLAB (R2015a)	MATLAB	N/A
ImageJ	National Institutes of Health	https://imagej.nih.gov/ij/download.html
FibrilTool plug-in	Boudaoud et al., 2014	https://media.nature.com/original/nature-assets/nprot/journal/v9/n2/extref/nprot.2014.024-S3.txt
Ingenuity Pathway Analysis software	QIAGEN	N/A
Enrichr	Enrichr - Ma’ayan Laboratory - Computational Systems Biology	http://amp.pharm.mssm.edu/Enrichr/
Columbus Image Data Storage and Analysis System	PerkinElmer	http://www.perkinelmer.com/es/product/image-data-storage-and-analysis-system-columbus
Search-Based Exploration of Expression Compendium (SEEK)	Troyanskaya Functional Genomics Laboratory at Princeton University, 2014	http://seek.princeton.edu/
GraphPad Prism	GraphPad Software	https://www.graphpad.com/company/
Fisher exact test calculator	Social Science Statistics web site	http://www.socscistatistics.com/tests/fisher/Default2.aspx
Other
pre-printed micropatterns	CYTOO	N/A
Fibronectin-coated 6-well plates for uniaxial stretching	FlexCell	Cat# UF-4001P
Flexcell FX-5000 Tension System	FlexCell	N/A
Protein G Sepharose 4 Fast Flow	GE Healthcare	Cat# GE17-0618-01