. Author manuscript; available in PMC: 2019 Aug 1.

Published in final edited form as: J Biophotonics. 2018 May 6;11(8):e201700352. doi: 10.1002/jbio.201700352

Table 1:

The summary of comparison of HPLC, Florescence AGE assay and Raman parameters across ribose-incubated groups. Significant p-values are in bold. N.S.: not significant.

Parameters			Glycation Treatment
			Ribose
			R-Control	R-0.1 M	R-0.5 M	R-Control vs. R-0.1 M	R-Control vs. R-0.5 M	R-0.1 M vs. R-0.5 M
HPLC Parameters	PEN/collagen	mol/mol of collagen	3.5±0.8	42.5±10.0	55.4±9.5	p<0.0001	p<0.0001	p<0.0001
	DPD/collagen	mol/mol of collagen	0.226±0.054	0.206±0.049	0.186±0.048	N.S.	N.S.	N.S.
	PYD/collagen	mol/mol of collagen	0.480±0.065	0.357±0.040	0.259±0.041	N.S.	N.S.	N.S.
Fluorescence AGE Assay	fAGEs	g/mol	76.2±24.9	456.0±81.2	829.3±138.2	p<0.0001	p<0.0001	p<0.0001
Raman Parameters	Mineral-to- Matrix (MMR)	ν₁PO₄/Amide I	26.93±2.10	26.56±1.55	26.72±3.12	N.S.	N.S.	N.S.
		ν₁PO₄/Amide III	10.82±1.56	11.30±1.19	11.20±1.82	N.S.	N.S.	N.S.
		ν₁PO₄/CH₂ wag	10.76±1.35	10.99±1.02	10.91±1.56	N.S.	N.S.	N.S.
		ν₁PO₄/Proline	16.67±1.30	16.88±0.75	16.35±1.22	N.S.	N.S.	N.S.
	Carbonate Substitution	CO₃/ν₁PO₄	0.148±0.005	0.150±0.007	0.147±0.006	N.S.	N.S.	N.S.
	Crystallinity	1/FWHM(ν₁PO₄)	0.063±0.007	0.062±0.001	0.063±0.006	N.S.	N.S.	N.S.
	Hyp/Pro	877/855	0.76±0.05	0.77±0.04	0.83±0.05	N.S.	p=0.0287	p=0.0288
	Hyp/Pro	877/922	0.75±0.05	0.74±0.02	0.82±0.05	N.S.	p=0.021	p=0.020
	Raman-PEN	~1362/CH₂-wag	0.518±0.112	0.468±0.081	0.512±0.172	N.S.	N.S.	N.S.
	Raman-PEN	~1495/CH₂-wag	0.164±0.048	0.149±0.040	0.156±0.064	N.S.	N.S.	N.S.
	Raman-CML	~1150/CH₂-wag	0.048±0.021	0.045±0.013	0.049±0.023	N.S.	N.S.	N.S.