. Author manuscript; available in PMC: 2019 Aug 1.

Published in final edited form as: J Biophotonics. 2018 May 6;11(8):e201700352. doi: 10.1002/jbio.201700352

Table 2:

The summary of comparison of HPLC, Florescence AGE assay and Raman parameters across glucose-incubated groups. Significant p-values are in bold. N.S.: not significant.

Parameters			Glycation Treatment
			Glucose
			G-Control	G-0.1 M	G-0.5 M	G-Control vs. G-0.1 M	G-Control vs. G-0.5 M	G-0.1 M vs. G-0.5 M
HPLC Parameters	PEN/collagen	mol/mol of collagen	2.9±1.1	3.1±1.1	4.2±1.2	N.S.	p=0.010	p=0.011
	DPD/collagen	mol/mol of collagen	0.241±0.052	0.226±0.071	0.217±0.056	N.S.	N.S.	N.S.
	PYD/collagen	mol/mol of collagen	0.423±0.090	0.398±0.088	0.408±0.091	N.S.	N.S.	N.S.
Fluorescence AGE Assay	fAGEs	g/mol	64.4±27.9	96.9±83.3	101.3±73.3	N.S.	N.S.	N.S.
Raman Parameters	Mineral-to-Matrix	ν₁PO₄/Amide I	27.63±2.03	28.07±2.74	26.52±2.37	N.S.	N.S.	N.S.
		ν₁PO₄/Amide III	13.64±0.74	13.88±1.02	13.98±0.74	N.S.	N.S.	N.S.
		ν₁PO₄/CH₂ wag	13.26±0.64	13.43±1.00	13.59±0.89	N.S.	N.S.	N.S.
		ν₁PO₄/Proline	17.67±1.03	17.41±0.50	16.99±0.81	N.S.	N.S.	N.S.
	Carbonate Substitution	CO₃/ν₁PO₄	0.138±0.005	0.139±0.006	0.142±0.005	N.S.	N.S.	N.S.
	Crystallinity	1/FWHM(ν₁PO₄)	0.066±0.003	0.065±0.001	0.064±0.001	N.S.	N.S.	N.S.
	Hyp/Pro	877/855	0.73±0.01	0.75±0.02	0.76±0.02	N.S.	p=0.0069	N.S.
	Hyp/Pro	877/922	0.68±0.02	0.72±0.01	0.75±0.04	N.S.	p=0.0039	N.S.
	Raman-PEN	~1362/CH₂-wag	0.335±0.016	0.331±0.037	0.295±0.049	N.S.	N.S.	N.S.
	Raman-PEN	~1495/CH₂-wag	0.077±0.008	0.077±0.015	0.070±0.014	N.S.	N.S.	N.S.
	Raman-CML	~1150/CH₂-wag	0.030±0.005	0.031±0.009	0.027±0.010	N.S.	N.S.	N.S.