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. 2018 Nov 9;6:e5905. doi: 10.7717/peerj.5905

Figure 1. Primer design and optimization of DRBD3 based qPCR for parasite quantification.

Figure 1

(A) Multisequence alignment based on 11 homologous sequences to L. major Sd. DRBD3 found using NCBI Blast. (B) Primer sequences and cycling parameters used. (C) DRBD3 primers amplify a 140 bp product specifically. Product visualized with ethidium bromide staining of a 1% agarose gel run and compared to New England BioLabs 100 bp ladder. (D) DRBD3 primers amplify diverse Leishmania spp. A representative plot of Ct value vs log dilution of parasite burden is shown. The average primer efficiency (±standard error of the mean) is indicated in parentheses for the following species: L. major Seidman (n = 6), L. major Friedlin (n = 5), L. venezuelensis (n = 5), L. panamensis (n = 3), and L. donovani (n = 3).