Table 4.
Rates of false negatives (species not detected) and false positives (species detected but not included in the mock communities) for the four fragments in all libraries
| Library | False negatives % | False positives (contamination) % | |||
|---|---|---|---|---|---|
| 18S | FC | Leray | Folmer | ||
| 1a | 40.00 | 0.43 | 1.90 | 0.15 | 0.16 |
| 1b | 31.25 | 4.90 | 0.11 | 0.34 | 0.05 |
| 1c | 28.57 | 6.09 | 36.24 | 94.16 | 9.91 |
| 1d | 25.00 | 4.96 | 16.12 | 80.79 | 93.51 |
| 1e | 28.57 | 3.59 | 32.18 | 57.95 | 7.38 |
| 1 g | 7.41 | 0.22 | 0.06 | 0.02 | 0.06 |
| SIS Average | 26.80 | 3.36 | 14.43 | 38.90 | 18.51 |
| 2a | 0.00 | 3.52 | 1.61 | 0.05 | 0.54 |
| 2b | 20.00 | 0.15 | 0.09 | 0.13 | 0.11 |
| 2c | 0.00 | 0.24 | 0.21 | 1.60 | 0.02 |
| 2d | 0.00 | 0.40 | 0.20 | 0.11 | 0.07 |
| 2e | 7.14 | 1.89 | 0.73 | 0.01 | 0.21 |
| 2 g | 11.54 | 0.34 | 0.03 | 0.02 | 0.05 |
| MIS Average | 6.45 | 1.09 | 0.48 | 0.32 | 0.17 |
| 3a1 | 0.00 | 0.07 | 14.96 | 51.59 | 16.62 |
| 3a2 | 0.00 | 0.01 | 0.18 | 0.09 | 0.02 |
| 3a3 | 0.00 | 0.01 | 0.17 | 0.07 | 0.02 |
| 3b1 | 0.00 | 0.12 | 0.02 | 0.05 | 0.09 |
| 3b2 | 0.00 | 6.95 | 9.01 | 0.35 | 0.52 |
| 3b3 | 0.00 | 0.23 | 0.12 | 0.04 | 60.00 |
| 3c1 | 0.00 | 0.48 | 2.02 | 2.13 | 0.41 |
| 3c2 | 0.00 | 0.41 | 36.36 | 45.27 | 8.61 |
| 3c3 | 0.00 | 0.55 | 0.41 | 0.81 | 0.21 |
| 3d1 | 0.00 | 80.77 | 0.04 | 0.08 | 100.00 |
| 3d2 | 0.00 | 96.99 | 0.01 | 0.09 | 0.05 |
| 3d3 | 0.00 | 75.76 | 0.02 | 0.12 | 0.04 |
| PPS Average | 0.00 | 21.86 | 5.28 | 8.39 | 15.55 |