Table 1.
Quantification of serotonin in brain areas involved during the MGT
| ASD | 5-HT | 5-HIAA | Ratio 5-HT | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Control | P value | ASD | Control | P value | ASD | Control | P value | ||
| OFC | 796 ± 55.7 | 1174 ± 85.7 | *p = 0.01 | 576 ± 38.6 | 1006 ± 78.4 | *p = 0.001 | 0.72 ± 0.02 | 0.85 ± 0.02 | *p = 0.03 |
| PrL/IL | 717 ± 21.2 | 1583 ± 123.7 | p = 0.42 | 1453 ± 40.5 | 667 ± 100.4 | p = 0.43 | 2.03 ± 0.04 | 2.13 ± 0.02 | p = 0.39 |
| Cins | 907 ± 28.5 | 800 ± 12.7 | *p = 0.04 | 679 ± 31.0 | 609 ± 28.4 | p = 0.28 | 0.74 ± 0.02 | 0.76 ± 0.02 | p = 0.78 |
| Amy | 1359 ± 47.9 | 1369 ± 92.2 | p = 0.92 | 923 ± 49.4 | 864 ± 43.2 | p = 0.55 | 0.67 ± 0.02 | 0.63 ± 0.01 | p = 0.38 |
| Hippocampus | 897 ± 38.7 | 1259 ± 78.9 | *p = 0.0007 | 1536 ± 74.9 | 1134 ± 27.8 | *p = 0.001 | 1.72 ± 0.06 | 0.90 ± 0.05 | *p < 0.0001 |
| CPu | 1579 ± 61.2 | 1466 ± 90.3 | p = 0.38 | 1072 ± 43.2 | 1169 ± 94.2 | p = 0.31 | 0.68 ± 0.01 | 0.79 ± 0.06 | *p = 0.001 |
| *p < 0.05 | |||||||||
Serotonin (5-HT), 5-HIAA, and 5-HT turnover (5-HT/5-HIAA) levels (mean ± SEM) in the OFC, Pre and Infra limbic (PrL/IL), CIns, Amy, hippocampus, and CPu for control animals (n = 3) and sleep debt animals (ASD, n = 12, *p < 0.05). ASD animals exhibited lower 5-HT, 5-HIAA, and 5-HT turnover in the OFC; higher 5-HT level in the CIns, lower 5-HT level in the hippocampus but higher 5-HIAA and 5-HT turnover and lower 5-HT turnover in the CPu (*p <0.05 in bold).