Figure 1.
Schematic of the 16 day experiment for mice that experienced no SF (control) and for mice in which daily fragmentation of sleep occurred from 0800 to 2000 hr (red bars). C57BL/6J (B6) and B6.BKS(D)-Leprdb/J (db/db) mice experienced either SF or no SF. Prior to day zero (abscissa), all mice were acclimated for 7 days to each treatment condition. On day zero, mice were anesthetized and bilateral, dorsal skin biopsy (wounding) was made through the subcutaneous muscle (panniculus carnosus). On alternate days after the skin biopsy, the mice were weighed, blood glucose was measured, and the wounds were photographed (Figure 2). On day 16, mice were euthanized and wound tissue was collected.