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. 2018 Oct 26;12:381. doi: 10.3389/fncel.2018.00381

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Copyright © 2018 Czeredys, Vigont, Boeva, Mikoshiba, Kaznacheyeva and Kuznicki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of mRNA that encode store-operated calcium entry (SOCE) proteins in day in vitro 21 (DIV21) medium spiny neuron (MSN) cultures from YAC128 and wildtype mice. (A) Gene expression analysis of mRNA that encode SOCE proteins and proteins that are involved in Ca²⁺ homeostasis in MSNs from wildtype (control) and YAC128 mice using real-time polymerase chain reaction (PCR). The gene expression results for all genes were normalized to Gapdh. Data from wildtype MSNs were normalized to 1. (B) Relative mRNA levels of genes that encode SOCE proteins, measured as 2^∧-dCt. (C) Immunoblots of HTT-associated protein-1 (HAP1) normalized to GAPDH and CacyBP/SIP (both monomers and dimers) normalized to Vinculin in YAC128 and wildtype MSN cultures. On the bottom of each panel, the expression levels of HAP1 and CacyBP/SIP proteins are plotted. The results are expressed as mean ± SEM and represent data from three independent mRNA and protein preparations of three different MSN cultures. ns, not significant.