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. 2018 Nov;11(11):1594–1606. doi: 10.1016/j.jcmg.2017.09.020

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Overview of the Experimental Protocol

(A) Protocol for the isolated perfused heart experiments. After a stabilization period, hearts were assessed with carbon-13 (orange) and phosphorus-31 (blue) MRS at baseline, immediately after a 20-min period of total global ischemia, and after 45 min of reperfusion. Buffer samples were collected for LDH measurements (green). After the final carbon-13 scan, hearts were freeze-clamped for subsequent messenger RNA analyses. (B) In vivo experimental protocol. Carbon-13 MRS and MRI experiments were performed 1 day after cryo-induction of MI. MRS experiments were repeated after 7 days. LDH = lactate dehydrogenase; MI = myocardial infarction; MRI = magnetic resonance imaging; MRS = magnetic resonance spectroscopy.