Fig 2. Non-polymerizable mutant actin efficiently activates VnExoY but is impaired in the activation of PaExoY.

Non-polymerizable actin from D. melanogaster (NP-actin at 0.1, 0.5 or 0.7 μM) or the corresponding wild-type control (Dm-actin at 0.1, 0.5, 0.7, 1.0 or 3.0 μM) were used to activate 1 ng PaExoY (A) or 10 ng VnExoY (B). Reactions were performed in reaction buffer containing 50 mM KCl and started by the addition of 2 mM substrate (ATP or GTP) and incubated for 30 or 10 min at 30°C for VnExoY or PaExoY, respectively. Error bars correspond to s. d. of two independent experiments.