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3T3-L1 preadipocytes were differentiated in D-MEM (1-methyl 3-isobutylxanthine, dexamethasone, and insulin) medium for 7 days. Triglyceride accumulation visualized by Oil-Red O staining. Mature adipocytes show red large lipid vacuoles that occupied most of the cytoplasm. Original magnification: 400x.
