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. 2018 Nov 12;13(11):e0207205. doi: 10.1371/journal.pone.0207205

Fig 5. Effect of SNX5 S226 phosphorylation on macropinocytosis.

Fig 5

8505C cells were treated with control or SNX5 siRNA for 48 h and rescue-SNX5-FLAG WT, S226A and S226E were then expressed for 24 h by adenovirus infection. (A) Cell lysates were immunoblotted with anti-FLAG, anti-SNX5 and anti-GAPDH antibodies. (B) After the rescue proteins had been expressed, each of the cell preparations were incubated with 500 μg/mL of FITC-conjugated 70 kDa dextran in the presence of 100 ng/mL EGF for 15 min. (C) The number of vesicles which containing FITC-dextran were counted in each cell. Data for 68 cells from three independent experiments are represented as the mean ±SEM. Asterisk indicates; p<0.001 by Student’s t-test.