Fig 7. Sepsis-induced NK-cell intrinsic functional impairment is associated with reduced DAP12 adaptor protein expression and clustering.

(A) Experimental Design. Splenic NK-cells were purified by negative selection 2 days post-surgery and stimulated as indicated. Immunoblot (B) and ratio quantification (C) for DAP12 and GAPDH in unstimulated NK-cells from Sham and CLP mice, numbers indicate replicates. (D) Ratio of cells obtained from Sham or CLP mice adhering to the glass slide versus non-adherent cells. (E) Representative images of DAP12 staining for TIRF microscopy. (F) Mean DAP12 pixel intensity of adhered cells. Each data set is representative from 2 independent experiments with 5x10⁵ cells per lane in panels B,C; 3 mice per group in panel D; >80 cells were analyzed per group from 3 mice per group in panels E,F. * p<0.05. Error bars represent the standard error of the mean.