Fig 12. IL-2c therapy does not improve DAP12 expression or intrinsic NK-cell function.

(A) Experimental Design. One day after surgery mice were treated with IL-2c or IgG (control). Cytokine production in response to receptor stimulation and DAP12 expression of NK-cells was determined 6 days later. (B) Frequency of IFN-γ⁺ of stimulated receptor⁺ NK-cells from Sham or CLP mice after 8 hrs stimulation with plate bound control (IgG), αLy49H, or αLy49D antibody. Immunoblot (C) and ratio quantification (D) for DAP12 and GAPDH in unstimulated NK-cells from Sham and CLP mice, numbers indicate replicates. Data are representative from 2 independent experiments with 4–5 mice per group in panel B and 1 independent experiment with 5x10⁵ cells per lane in panels C,D. * p<0.05. Error bars represent the standard error of the mean.