S1 (0pg/µl)	S2 (33.3pg/µl)	S3 (100pg/µl)	S3 (300pg/µl)	S5 (600pg/µl)	S6 (1200pg/µl)
standard protein(1200pg/µl)	0	1.39	4.17	12.5	25	50
IgG free BSA (4pg/µl)	50	48.61	45.83	37.5	25	0
total	50	50	50	50	50	50