X1 (0µg/µl)	X2 (0.25µg/µl)	X3 (0.5µg/µl)	X4 (1µg/µl)	X5  (2µg/µl)	X6  (4 µg/µl)
Sample(4µg/µl)	0	3.125	6.25	12.5	25	50
IgG free BSA(4µg/µl)	50	46.875	43.75	37.5	25	0
total	50	50	50	50	50	50