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. 2018 Oct 29;7:e38829. doi: 10.7554/eLife.38829

Figure 3. Postsynaptic receptors to DA9 maintain their localization and are aligned with DA9 SV clusters after axotomy.

(A) Labeling of DA9 axon and ACR-12 in GABA motor neurons in intact animals (2d old adults). Scale bars = 10 μm. (B) Labeling of DA9 axon and ACR-12 in GABA motor neurons in axotomized animals. Scale bars = 10 μm. (C) ACR-12::GFP puncta number in the putative DA9 synaptic region (~85 μm anterior from the most posterior ACR-12 puncta in the dorsal nerve cord) in both intact and axotomized animals. ns, not significant. Unpaired t test. (D) Length of posterior DA9 axon without ACR-12 in both intact and axotomized animals. ns, not significant. Unpaired t test. (E) BFP::RAB-3 puncta in DA9 axon appose ACR-12::GFP puncta in the postsynaptic GABA motor neurons in intact animals. Scale bars = 10 μm. (F) BFP::RAB-3 puncta in DA9 axon appose ACR-12::GFP puncta in the postsynaptic GABA motor neurons 48 hr after axotomy. Scale bars = 10 μm. (G) Percentage of BFP::RAB-3 apposing ACR-12::GFP in intact and axotomized animals. ns, not significant. Unpaired t test.

Figure 3.

Figure 3—figure supplement 1. No change of postsynaptic receptors in body wall muscles after axotomy.

Figure 3—figure supplement 1.

(A–A’’) Muscle ACR-16 localization in intact animals. Note its localization in both the dorsal and ventral nerve cords. Scale bars = 10 μm. (B–B’’) Muscle ACR-16 maintains its localization 48 hr after DA9 axotomy. Scale bars = 10 μm. (C) ACR-16::GFP puncta number in the putative DA9 synaptic region (~85 μm anterior from the most posterior ACR-16 puncta in the dorsal nerve cord) in both intact and axotomized animals. ns, not significant. Unpaired t test. (D) Length of posterior DA9 axon without ACR-16 in both intact and axotomized animals. ns, not significant. Unpaired t test. (E) Number of ACR-16::GFP puncta overlapping with DA9 dendrite in both intact and axotomized animals. ns, not significant. Unpaired t test.