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. 2018 Oct 29;7:e38829. doi: 10.7554/eLife.38829

Figure 8. JNK-1 and dynein-mediated transport mediate SV mislocalization to the dendrite and loss of JNK-1 improves behavioral recovery.

(A–D) GFP::RAB-3 localization 48 hr after axotomy in ric-7(n2657) (A), ric-7(n2657); dhc-1(js319) (B), ric-7(n2657); nud-2(ok949) (C) and ric-7(n2657); jnk-1(gk7) (D) animals. Asterisk indicates cell body and bracket indicates dendrite. Scale bars = 10 μm. (E) Quantification of the number of dendritic RAB-3 puncta in intact and axotomized animals 48 hr after axotomy. Mean ± SEM. **p<0.01; ****p<0.0001; ns, not significant. Unpaired t test. (F) Traces of the tail-bending behavior of ric-7(n2657) and ric-7(n2657); jnk-1(gk7) animals with and without axotomy 48 hr after axotomy. The shaded area indicates the 5 s stimulation. Mean ± SEM. (G) Averaged tail angle during stimulation of the animals in (F). Numbers represent the number of animals. Mean and SEM. ***p<0.001; ****p<0.0001. Unpaired t test.

Figure 8.

Figure 8—figure supplement 1. DA9 in dhc-1(js319) animals develops normally and loss of JNK-1 rescues RAB-3 mis-localization in DA9 dendrite 12 hr after axotomy.

Figure 8—figure supplement 1.

(A–B) RAB-3::GFP and mCherry labeling of DA9 in intact ric-7(n2657) and ric-7(n2657); dhc-1(js319) animals (2-day-old adults). Scale bars = 10 μm. (C–F) RAB-3 localization to the dendrite inArxiocn-7ti(pn2657) and ric-7(n2657); jnk-1(gk7) animals with and without axotomy 12 hr after axotomy. Magnified GFP channels are shown at the top right corner. Scale bars = 10 μm. (G) Quantification of dendritic RAB-3 puncta number in animals in (C–F). Mean ± SEM. *p<0.05; ****p<0.0001; ns, not significant. Unpaired t test.