Table 1.
Details | Drug assay | |||
|
IDKmonitor | LISA TRACKER (Infliximab) | Promonitor (Infliximab) | RIDASCREEN |
Microplate coating | Anti-IFXa monoclonal antibody | Recombinant TNFb alpha | Anti-TNF alpha monoclonal antibody bound to recombinant TNF alpha | TNF alpha |
Primary conjugate | HRPc-conjugated anti-IFX monoclonal antibody | Biotinylated antihuman IgG1d antibody | HRP-conjugated anti-IFX monoclonal antibody | HRP-conjugated anti-IFX monoclonal antibody |
Secondary conjugate | —e | Streptavidin-HRP conjugate | — | — |
Detection method | TMBf | TMB | TMB | TMB |
Measurement range (µg/mL) | 0.4-45.0 | 0.3-16.0 | 0.035-14.4 | 0.1-12.0g |
aIFX: infliximab.
bTNF: tumor necrosis factor.
cHRP: horseradish peroxidase.
dIgG1: immunoglobulin G subclass 1.
eTMB: tetramethylbenzidine
fNot applicable.
g(2.0-48.0 on dilution).