Table 2.

Antidrug antibody assay kit specifications.

Details	Antidrug antibody assay
	IDKmonitor (total)a	LISA TRACKER (free)	Promonitor (free)	RIDASCREEN (free)
Microplate coating	Streptavidin	IFXb	IFX	IFX
Primary conjugate	HRPc-conjugated IFX	Biotinylated-IFX	HRP-labelled IFX	Biotinylated-IFX
Secondary conjugate	Biotinylated-IFX	Streptavidin-HRP conjugate	—d	Streptavidin-HRP conjugate
Detection antibody	TMBe	TMB	TMB	TMB
Measurement range	10 arbitrary unit/mLf	10-200 ng/mL	2-1440 arbitrary unit/mL	2.5-125 ng/mLg

^aSamples are subjected to manual infliximab-antidrug antibody dissociation step.

^bIFX: infliximab

^cHRP: horseradish peroxidase

^dNot applicable.

^eTMB: tetramethylbenzidine.

^fSemiquantitative, single cutoff.

^g(20-1000 ng/mL extended range).