Method	Sample volume [mL]	# of proteins identified	Comments
FASP	1	227	bulk of sucrose precipitated on filter
acetone precipitation	0.6	372	viscous sludge of sucrose at the tube bottom; 4:1 (reagent:sample) volumes; no visible protein pellet
chloroform/methanol precipitation	0.32	389	viscous sludge of sucrose at the tube bottom; 8:1 (reagent:sample) volumes; no visible protein pellet
TCA precipitation (at 4 °C)	0.5	405, 415	no sucrose precipitation; 0.12:1 (reagent:sample) volumes; visible pellet at the tube bottom
re-precipitation		85, 60	additional TCA precipitation of supernatant after first pellet was collected
TCA precipitation (at -20 °C)	0.5	440, 430	no sucrose precipitation; 0.12:1 (reagent:sample) volumes; visible pellet at the tube bottom
re-precipitation		81, 55	additional TCA precipitation of supernatant after first pellet was collected