FRACTIONS | GENE Ct | LUCIFERASE Ct | ΔCt | 2ΔCt | SUM OF ALL FRACTIONS | % OF EACH FRACTION | Polysome/NTR Ratio |
High efficiency (HMW) | 28.75 | 22.79 | 5.96 | 0.016 | 0.046 | 34.64 | 2.77 |
Low efficiency (LMW) | 28.43 | 22.63 | 5.80 | 0.018 | 38.81 | ||
Non Translating (NTR) | 29.12 | 22.77 | 6.35 | 0.012 | 26.55 | ||
Polysome/NTR Ratio = (HMW + LMW)/NTR |