| Find the SCM minimum value: 22.77 | |||
| Sample 1 | Sample 2 | Sample 3 | |
| Ct MOI | 22.92 | 22.36 | 22.58 |
| Ct SCM | 22.81 | 22.77 | 22.9 |
| Ct REF | 23.27 | 23.55 | 23.19 |
| Normalize all the SCM values to this minimum | |||
| Sample 1 | Sample 2 | Sample 3 | |
| Ct SCM | 0.05 | 0 | 0.13 |
| Subtract these values from the Ct values of MOI and REF | |||
| Sample 1 | Sample 2 | Sample 3 | |
| Ct MOI | 22.87 | 22.36 | 22.45 |
| Ct REF | 23.22 | 23.55 | 23.06 |
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