Figure 3. Early handling prevents de novo DNA methylation of Oxtr.
Oxtr DNA methylation levels at CpG sites (A) −934_1, (B) −934_2, (C) −924, and (D) −901 in the forebrain of ED21 offspring (n=18, 9 male/female sibling pairs) are similar to MAN1 offspring that received increased early parental care, whereas MAN0 offspring that received low amounts of early care have a significant increase in postnatal de novo DNA methylation in the nucleus accumbens (unpaired two-tailed t test; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001; error bars indicate standard error).