Table 1.
LV morphology and in vivo cardiac performance measured by echocardiography and pressure-volume loop analysis.
| Echocardiography assessment | ||||
| WT (n = 7) |
MLP−/− (n = 7) |
MYBPC3−/− (n = 7) |
DKO (n = 7) |
|
| LVmass/BW(mg/g) | 4.99 ± 0.30 | 6.64 ±0.31* | 11.91 ±0.66*,# | 6.25 ± 0.48* |
| HR (bpm) | 497 ± 16 | 509 ± 21 | 471 ± 11 | 446 ± 25 |
| PWd (mm; | D.93 ± 0.05 | 0.71 ± 0.03*,# | 1.29 ± 0.07* | 1.18 ± 0.05* |
| PWs (mm) | 1.43 ± 0.07 | 0.88 ± 0.04*,# | 1.53 ± 0.06 | 1.46 ± 0.05 |
| ESD (mm) | 2.17 ± 0.04 | 3.87 ± 0.10*,# | 3.34 ± 0.14*,# | 2.38 ± 0.12 |
| EDD (mm) | 3.43 ± 0.09 | 4.52 ± 0.12*,# | 4.18 ± 0.17*,# | 3.36 ± 0.16 |
| FS (%) | 36.62 ± 0.91 | 14.45 ± 1.67*,# | 20.05 ± 1.16*,# | 29.01 ± 1.91* |
| EF (%) | 67.40 ± 1.17 | 30.60 ±3.11*,# | 41.28 ± 2.01*,# | 56.57 ± 2.86* |
| Pressure-volume loop analysis | ||||
| WT (n = 9) |
MLP−/− (n = 6) |
MYBPC3−/− (n = 6) |
DKO (n = 6) |
|
| HR (bpm) | 394 ± 17 | 412 ± 29 | 434 ± 17 | 408 ± 13 |
| ESP (mmHg) | 99.75 ± 3.41 | 85.07 ± 2.69* | 88.86 ± 5.92 | 80.39 ±3.08* |
| EDP (mmHg) | 5.53 ± 0.49 | 9.70 ± 1.66* | 9.56 ± 1.37* | 10.53 ± 1.98* |
| ESV (μL) | 15.19 ± 2.00 | 27.61 ± 1.90*,# | 26.89 ± 1.59*,# | 16.28 ± 1.21 |
| EDV (μL) | 24.49 ± 1.41 | 36.54 ± 1.46*,# | 32.79 ± 1.36*,# | 23.31 ± 1.38 |
| EF(%) | 46.57 ± 5.86 | 31.08 ±3.61*,# | 26.84 ±3.99*,# | 39.58 ±4.19 |
| dP/dt max(mmHg/s) | 7174.2 ± 517.8 | 4496.4 ± 196.8*,# | 8166.3 ± 534.5 | 7851.0 ± 504.8 |
| td (ms) | 47.50 ± 3.21 | 43.30 ± 3.49 | 25.89 ± 1.55* | 28.10 ± 1.10* |
| ET (ms) | 53.30 ± 2.62 | 54.25 ± 2.82*,# | 38.98 ± 1.60*,# | 48.05 ± 2.31 |
| IVRT (ms) | 24.55 ± 1.71 | 34.74 ±2.28* | 33.24 ±2.71* | 33.03 ± 1.42* |
| T (ms) | 6.90 ± 0.40 | 10.61 ± 0.49* | 9.87 ± 0.45* | 10.32 ± 0.32* |
| S:D | 0.71 ± 0.03 | 0.93 ±0.11*,# | 0.51 ± 0.02* | 0.61 ± 0.02 |
Echocardiography assessment: BW, body weight; LV Mass/BW, ratio of LV and body weight; HR, heart rate; PWd, posterior wall thickness in diastole; PWs, posterior wall thickness in systole; ESD, end systolic diameter; EDD, end diastolic diameter; FS, fractional shortening; EF, ejection fraction. Pressure-volume loop analysis: HR, heart rate; ESP, end systolic pressure; EDP, end diastolic pressure; ESV, end systolic volume; EDV, end diastolic volume; EF, ejection fraction; dP/dtmax, maximum rate of pressure development; td, time to dP/dtmax; ET, ejection time; IVRT, isovolumic relaxation time; τ, time constant of pressure relaxation; S:D, systolic to diastolic duration ratio. Values are expressed as mean ± SEM.
Significantly different from WT (p b 0.05).
Significantly different from DKO (p b 0.05).