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Tri-lineage in vitro differentiation assays of equine BM-MSCs at passage 3. Calcium deposition was stained by Alizarin Red S in the osteogenic treatment group (A). Intracellular neutral lipid droplets were detected by Oil Red O in the adipogenic treatment group (B). Alician blue was used to stain proteoglycans in the extracellular matrix of the chondrogenic differentiation group (C). (D,E,F) are the control group for osteogenic, adipogenic and chondrogenic, respectively.
