. 2018 Oct 9;5:31. doi: 10.21037/sci.2018.09.01

Table 1. The number of colonies on days 7 and 14 after seeding; approximate confluency and cell numbers on day 21.

Density (cells/cm²)	7^th day colonies (>15 cells)	14^th day colonies (>15 cells)	14^th day colonies (<15 cells)	21^st day approximate confluency (%)	21^st days adherent cell numbers
1×10⁵	1.5±0.5^a	4.5±0.5^a	8.5±3.5^a	6±1^a	80,000±10,000^a
2×10⁵	2.5±0.5^a	12.5±0.5^a	6.5±2.5^a	11.5±1.5^a	165,000±45,000^a
4×10⁵	4.5±0.5^a	68.5±25.5^a	76±18^a	16±1^a	1,600,000±300,000^b
8×10⁵	3.5±1.5^a	51±15^a	45±5^a	22.5±2.5^b	350,000±50,000^a
1×10⁶	10±3^a	130±28.5^b	135±21^b	37.5±7.5^b	1,100,000±400,000^a,b

Data are presented as mean ± SD. Values with different alphabets (a, b) within columns are significantly different (P<0.05).